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Serum creatinine (a blood measurement) is an important indicator of renal health because it is an easily measured byproduct of muscle metabolism that is excreted unchanged by the kidneys.

Creatinine itself is produced via a biological system involving creatine, phosphocreatine (also known as creatine phosphate), and adenosine triphosphate (ATP, the body's immediate energy supply).

A recent report also suggests that creatinine may have immunosuppressive properties.Each day, 1–2% of muscle creatine is converted to creatinine.Creatinine appears not to affect growth of fungi and yeast; this can be used to isolate slower growing fungi free from the normal bacterial populations found in most environmental samples.Many laboratories will automatically calculate e GFR when a creatinine test is requested.Algorithms to estimate GFR from creatinine level and other parameters are discussed in the renal function article.

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However, in cases of severe renal dysfunction, the Cr Cl rate will overestimate the GFR because hypersecretion of creatinine by the proximal tubules will account for a larger fraction of the total creatinine cleared.

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